Nov 11, 2020 · Lentivirus was packaged by transfecting Lenti-X™ 293T cells (Clontech Catalog #632180) with the lentivirus plasmid and helper plasmids (Cellecta Catalog #CPCP-K2A) using the lipofectamine 2000 reagent (Invitrogen Catalog #11668027). The viral supernatant was collected 72 hours post transfection, followed by virus concentration
The TIAO USB Multi Protocol Adapter (TUMPA) is a multi-functional USB communication adapter for hobbyists or engineers. The adapter is based on FDTI's flagship communication chip FT2232H, a USB 2.0 Hi-Speed (480Mb/s) to UART/FIFO IC. It has two multi-protocol synchronous serial engines...
Transfection was with Lipofectamine 3000 (Invitrogen) according to manufacturer's protocol. At 24 hours after transfection, cells were washed once in PBS and resuspended in PBS media containing 3% BSA Fraction V. The cell suspension was then filtered through a 35 mm membrane followed by immediate FACS sorting using the RFP+ selection marker.
P3000™ Reagent Lipofectamine® 3000 Reagent used. Genomic Cleavage Detection (GCD0.2μL. 0.15 and 0.3 μL. Overview of protocol outline for generating iPS cells with Epi5™ Episomal reprogramming kit and Lipofectamine® 3000 reagent.
transfected with packaging plasmids into 293T cells at the indicated concentrations using Lipofectamine 2000 (Invi-trogen Life Technologies) according to the manufacturer’s instructions, producing the lentivirus LV402-pL6.3-CMV-GFPa1-IRES-mus-TGF-β and the negative control PDS019.
Protocol. 1. Cell preparations in 96-well plates. For HEK293 cells transiently trasfected with human TRPA1 cDNA. Transfer an equal volume of the diluted Lipofectamine 2000 to the tube that contains the diluted cDNA, vortex and let the mixture sit at room P3000MP. Poly-L-ornithine. Sigma-Aldrich.
Jun 02, 2016 · In this protocol, we have demonstrated use of insect and mammalian cells for production of SVP or VLPs in supernatant and purification by ultracentrifugation. Four subtypes of DENV prM/E structural gene expression cassettes were used to construct the versions of DENV SVPs (demarcated as 1-4) in Table 1 and demonstrate a range between 1.1-2.6 mg ...